Recombinant DNA Technology
Recombinant DNA technology is used to mass-produce
proteins and genes inside bacteria.
The basic procedure used in this form of genetics engineering
is diagrammed in figure and described following.
The procedure begins by isolating the gene that
codes for protein to be mass-produced. The segment
of DNA containing the gene is removed from its natural
location, usually a human cell, and attached to a piece
of DNA that invades a bacterium. The attached gene is
the donor gene and the DNA molecule that receives
it the vector:
Together, the donor gene and vector are called recombinant
DNA; they are a combination of two kind of DNA.
Bacteria are used in recombinant DNA technology
because they are easy to grow in the laboratory, they reproduce
rapidly, and their mechanisms for turning genes
on and off are well understood. Moreover, bacteria readily
accept pieces of DNA from one another almost
piece of DNA placed inside a bacterium will be used to